RNA
Part:BBa_K4501000:Experience
Designed by: Pau Marín Escudero Group: iGEM22_Barcelona_UB (2022-08-22)
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Applications of BBa_K4501000
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. Results of qPCR for Ramos and HEK293T cell line.
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UNIQ574da2957a0a3bc2-partinfo-00000000-QINU UNIQ574da2957a0a3bc2-partinfo-00000001-QINU